1. WSSV The Current Scenario  2. Principle of Polymerase Chain Reaction (PCR)  3. Components of PCR 
4. Equipment Required for a PCR Laboratory 5. Agarose Gel Electrophoresis 6.. Sampling Strategy for PCR Protocol 7. Template DNA Preparation for PCR 8. Single Step PCR Protocol for WSSV 9. Nested PCR in WSSV Detection 10. PCR Protocol for Infectious Hypodermal and Hematopoeitic Necrosis Virus (IHHNV) 11. PCR Protocol for Yellow Head Virus (YHV) 12. PCR Protocol for Taura Syndrome Virus (TSV) 13. Protocol for Necrotising Hepatopancreatitis (NHP) 14. Modifications of Polymerase Chain Reaction 15. False Positives and False Negatives in PCR 16. Cause and Remedy for Troubleshooting in PCR 17. PCR in Diagnostic Biotechnology 18. PCR in Pathogenic Bacterial Detection 19. Setting up of a PCR Laboratory 20. Other Molecular Diagnostic Techniques 21. Electron Microscopy in Advanced Fisheries Research 22. Cryo Microscopy in Aquaculture Research