1. Safety Procedures in Biochemistry Laboratory 
2. Equipment inBiochemistry Laboratory 
3. Preparation of aqueous solutions 
4. Preparation of Standard Solutions 
5. Standardization of aqueous solution of acids and bases 
6. Determination of pH 
7. Preparation of Buffers 
8. Estimation of moisture content 
9. Determination of Ash Content (Total, Acid soluble and
Acid insoluble) 
10. Qualitative Determination of Carbohydrates 
11. Quantitative Estimation of carbohydrates in foods 
12. Determination of Crude Fiber 
13. Qualitative determination of protein 
14. Quantitative Estimation of protein in foods
15. Qualitative test of lipids 
16. Quantitative Estimation of lipids in foods 
17. Calculation of Calorific Value 
18. Determination of Quality Parameters of Lipids 
19. Spectrophotometry – Beers and Lambert law 
20. Gas Chromatography – Quantitative Analysis of Fatty
Acids 
21. High performance liquid chromatography (HPLC) –
Quantitative analysis of amino acid 
22. Electrophoretic separation of proteins by SDS