1. INTRODUCTION
2. CONVENTIONAL TECHNIQUES
I. Laboratory Techniques
II. Important Equipment in Microbiology
1. Methods of Sterilization and Disinfection
2. Preparation of Reagents & 
3. Staining of Bacterial Specimens
4. Aerobic Culture of Bacteria
5. Anaerobic Culture of Bacterial Cells
6. The Motility of Bacteria
7. Pure Culture Techniques
8. Colony Characteristics and Colony Counting
9. Biochemical Tests for Bacteria
10. Histopathological Techniques
11. Collection and Preservation of Infected Fish
12. Preparation of Blood Smear from Fish
13. Preparation of Wet Mount from Fish Samples
3. MOLECULAR TECHNIQUES
1. Polymerase Chain Reaction (PCR)
2. Genomic DNA Extraction from Tissue Samples
3. DNA Quality and Quantification
4. Gel Electrophoresis
5. SDS – Polyacrylamide Gel Electrophoresis
6. Enzyme-Linked Immunosorbent Assay (ELISA)
7. Blotting Techniques
8. Southern Blotting
Contents
9. Random Primer Labelling
10. Immunohistometry
11. IMMUNODOT
12. Microplastic Isolation from Fish
13. Heavy Metal Analysis in Fish Sample
14. Lethal Dose 50 (LD50) Calculation Method
15. Anatomical Procedure in Fish
16. Real-Time PCR
4. HAEMATO-IMMUNOLOGICAL TECHNIQUES
1. Determination of Erythrocyte Count.
2. Determination of Hemoglobin Levels
3. Haematocrit Value (HK, PCV)
4. Mean Corpuscular Volume (MCV)
5. Mean Corpuscular Hemoglobin (MCH)
6. Mean Corpuscular Hemoglobin Concentration (MCHC)
8. Leucocrit Value (LC, BC)
9. Aspartate Aminotransferase (AST)
10. Alanine Aminotransferase (ALT)
11. Total Protein (TP)
12. Cholesterol Levels
13. Urea
14. Triacylglycerol (TAG)
15. Glucose.
16. Prophenol Oxidase (PPO)
17. Superoxide Dismutase (SOD)
18. Respiratory Burst Activity (RBA)
19. Myeloperoxidase (MPO) Activity
20. Serum Lysozyme Activity
21. Phagocytic Activity
22. Total Immunoglobulin in Serum
23. Serum Ceruloplasmin Level
24. Albumin