PART-I: BIOTECHNOLOGY
1. Introduction to Biotechnology   2. Calculations in Molecular Biology and their Conversions   3. Molecular Biology Measurement Units   4. Scope of Biotechnology 
5. Importance of Biotechnology in Aquaculture/Fisheries   6. Structural Organization of Prokaryotic Cells   7. Molecular Biotechnology   8. Genetically Modified Organisms 
9. Model Organisms   10. Structural Organization of Eukaryotic Cells   11. Nucleic Acids Structure, Function and Types  12. Physico-chemical Properties of DNA 
PART-II: BIOTECHNOLOGY 
1. Genetic Code   2. Transcription  3. Translation   4. Mutations  5. Mutations and their Implications  6. Post-transcriptional Modifications   7. RNA Processing 
8. Gene Regulation  9. Gene Expression in Prokaryotes  10. Gene Regulation in Eukaryotes   11. DNA Sequencing   12. Lac Operons  13. Genetic Engineering 
14. Restriction Enzymes   15. Gene Isolation  16. Cloning Vectors 
PART-III: BIOTECHNOLOGY 
1. cDNA Synthesis and Total RNA Extraction   2. Cell Signaling and Signaling Molecules  3. Hyperchromicity  4. Molecular Events During Dna Replication  
5. Structure and Function of Dna Polymerase  6. Post-Transcriptional Modifications MCQs   7. Structure and Synthesis of tRNA and rRNA  8. Structure and Types of Ribosomes c
9. Mechanism of Initiation, Elongation, and Termination  10. Holliday Junction  11. DNA Repair- Enzymes Types and Mechnism  12. Wobble Hypothesis 
PART-IV: BIOTECHNOLOGY 
1. Probes   2. Recombinant Dna Technology  3. Vaccine Devlopment Using Rdna Technology  4. Transgenic Fishes  5. Gene Transfer Technology  6. Animal Cell Culture 
7. Hybridoma Technology  8. Molecular and Immunological Techniques  9. Polymerase Chain Reaction (PCR)  10. Immunoblotting  11. ELISA (Enzyme-Linked Immunosorbent Assay) 12. Principle of Hybridization  13. Northern Blotting  14. Western Blotting  14. Southern Blotting 
PART-V: BIOTECHNOLOGY
1. DNA Fingerprinting 2. Restriction Fragment Length Polymorphism (RFLP)  3. Bio-sensors  4. Bioremediation  5. Bioprocess Engineering and Bioprocessing
6. DNA Replication 7. Genome Editing Techniques  8. Gene Sequencing  9. Stem Cells 10. Molecular Markers   11. Industrial and Environmental Biotechnology 
PART-VI: BIOTECHNOLOGY
1. Cell Division  2. Cell Cycle  3. Cell Growth and Differentiation 4. Cell Trafficking and Signaling 5. Apoptosis and Molecular Pathways  6. Transposons and Intervening Sequences 7. Molecular Mechanisms of Spontaneous Mutations  8. Generation of DNA Fragments  9. Genetic Recombination, Transduction, Transformation and Conjugation  10. Cloning and Expression Vectors, Including Plasmids, Cosmids, Phages and Phagemids  11. Shuttle Vectors [BAC,YAC, MAC] 12. Site-Directed Mutagenesis 
13. In Situ Mutagenesis  14. Nano Delivery System 16. Micro RNAs  17. Reverse Genetics  18. RNA Interference (RNAi, siRNA, Peptide Nucleic Acid) 
PART-VII: BIOTECHNOLOGY 
1. Bioinformatics  2. Conservation, Storage and Maintenance of Cell Lines  3. Softwares used in Biotechnology  4. Data Analysis Tools for SNPs and ESTs  5. Biological Databases and Tools  6. Types of Biological Databases  7. Primary and Secondary Databases  8. Protein Data Bank  9. NCBI (National Center for Biotechnology Information) 
10. Formats and Contents in Bioinformatics  11. Sequence Retrieval  12. Manipulation in Bio-informatics  13. Primer Design  14. Restriction Mapping  15. Open Reading Frame 
16. European Molecular Biology Open Software Suite [EMBOSS] 17. Molecular Visualization and Sequence Analysis  Calculations for Molecular Biology and Biotechnology
Appendices