1. Introduction to Biotechnology 2. DNA as Genetic Material, Chemistry of Nucleic Acids  3. Organization of Genome in Prokaryotes and Eukaryotes 4. Concepts of Gene and Genetic Code 5. Translation 6. Post-Translational Modifications 7. Mutation and its Implications 8. Gene Expression and Regulation of Prokaryotes and Eukaryotes 9. DNA Replication 10. Transcription 11. Post Transcriptional Modification and RNA Processing 12. Enzymes Used in the Recombinant DNA Technology 13. Vectors Used in Recombinant DNA Technology 14. cDNA CLONIN 15. Probe Hybridization 16. Hybridoma Technology17. Vaccination 18. Genetically Modified Fish 19. Transgenic Fish Production 20. Animal Cell Culture 21. Immunological Techniques 22. ELISA (Enzyme Linked Immuno Sorbent Assay) 23. Molecular Techniques 24. Polymerase Chain Reaction (PCR) 25. DNA Sequencing 26. Western Blotting 27. Northern Blotting 28. Southern Blotting 29. DNA Fingerprinting 30. Marine Biotechnology 31. Biosensor 32. Waste Water Treatment 33. Bioremediation in Aquaculture Systems 34. Bioprocessing 35. Biofertilizer 36. Probiotics 37. Bioprospecting 38. Introduction to Bioinformatics 39. Primer Designing 40. Sequence Retrieval and Manipulation 41. Sequence Analysis 42. Sequence Similarity Searching 43. Phylogenetic Analysis 44. Molecular Visualization 45. ORF Finding 46. Restriction Mapping