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Techniques and Protocols in Plant Biochemistry and Molecular Biology

Techniques and Protocols in Plant Biochemistry and Molecular Biology

by Ranjan, R

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  • Year: 2015
  • Paperback/Hardbound: Hardbound
  • ISBN: 9789384337100
  • Language: ENGLISH

1. Protein Assay Chemistry 2. Protein Assay Data Analysis .3. Biuret Assay 4. Lowry Method for Protein Quantitation. 5. Bradford Method for Protein Quantitation 6. Bicinchoninic Acid (BCA) Protein Assay  7. Non-Interfering Protein Assay 8. Native Polyacrylamide Gel Electrophoresis (PAGE) 9. SDS-Polyacrylamide Gel Electrophoresis 10. Isoelectric Focusing 11. Two Dimensional Gel Electrophoresis 12. Maldi-TOF/TOF MS 13. Isolation of Genomic DNA from Plants (CTAB Method)  8314. Miniprep Protocol for Isolation of DNA from Plants .15. RNA Isolation and Its Analysis (TriZol Method) .16. Restriction Digest of DNA.17. Blotting Methods.18. Southern Blotting 19. Northern Blotting 20. Western Blotting.21. Polymerase Chain Reaction (PCR) 22. Reverse Transcription Polymerase Chain Reaction (RT-PCR) 23. Elution of DNA Fragments from Agarose Gel 24. Cloning of Gene.25. Plasmid Isolation (Alkaline Lysis Method) .26. Plasmid Isolation by using Column.27. In-Silico Characterization of Gene/Protein 28. Expression Analysis of Gene by Quantitative Real-Time PCR 29. Key Concepts 30. Appendix