1. Microscopy Techniques 2. Determination of Floc Volume.3. Determination of Specific Surface Area (SSA) of Floc 4. Dehydrogenase Activity Measurement (DAM) by Triphenyl Tetrazolium Chloride. 5. Determination of Floc Volume Index (FVI) 6. Determination of Floc Density Index (FDI) 7. Determination of Porosity 8. Determination of Floc Settling Velocity (FSV) 9. Determination of Floc Pore Radius (FPR) 
10. Determination of Floc Size .11. Determination of Total Suspended Solids (TSS) 12. Determination of Total Dissolved Solids (TDS) .13. Determination of Total Solids (TS) 14. Determination of Fixed and Volatile Solids (VSS) 15. Determination of Settleable Solids .16. Determination of Biochemical Oxygen Demand (BOD) 17. Determination of Organic Carbon (OC) 18. Determination of Sludge Volume Index (SVI) and Sludge Density Index (SDI) 19. Determination of Settled Sludge 20. Determination of Floc Strength 21. Determination of Floc Strength by CST (Capillary Suction Time) Test 22. Determination of EDTA- Test for Flocculation Mechanism 23. Determination of Surface Potential as Negative Surface Charge 24. Determination of Surface Energy as Internal Hydrophobicity 25. Determination of Chlorophyll  26. Determination of Sulphur Oxidation Test (S-Test) 27. Determination of ‘Anthrone Test’ for Total Carbohydrate .28. Determination of ‘Modified Lowry Method’ for Protein 29. Determination of Exocellular Biopolymer Extraction Methods 30. Filamentous Organism Identification Worksheet  31. Determination of Stirred Sludge Volume Index at SS Concentration of 3.5 G/L (SSVI3.5)  32. Determination of Diluted SVI (DSVI) Method 33. Determination of Foaming Test for High Solids Sample Analysis 34. Determination of Alka-seltzer® Foaming Test Culture for Biofloc Water 35. Determination of Loss on Ignition and Residue on Ignition 36. Determination of Total Kjeldahl Nitrogen 37. Determination of Sludge Concentration 38. Determination of Hydrogen Sulphide 39. Determination of Phosphorus 40. Determination of Potassium 41. Determination of Floc Shape 42. Extraction of Polyhydroxybutyrate (PHB) .43. Determination of Floc Breakage 44. Determination of Flocculating Ability 45. Determination of Floc Size Distribution 46. Determination of Fractal Dimension .47. Determination of Filament Index 48. Calculation of Net C/N atios of Feed .49. Calculation of Feed Economics of Aquaculture with and without, Biofloc 50. Calculation of Carbon Requirement