1. Study of microscope and its handling. 
2 Determination of characteristics of microorganisms by staining techniques
3 Sterilization techniques used in microbiology laboratory 
4 Preparation of culture media and their composition 
5 Isolation and purification of microorganisms from soils 
6 Isolation and enumeration of microorganisms from soil by Serial dilution technique-Pour plate method
7 Isolation and enumeration of spore forming bacteria from soil by Spread plate technique
8 Total counts of soil microorganisms by direct microscopic examination of soils
9 Viable bacterial counts in soil samples by serial dilution pour plate method
10 Study of legume root nodules and isolation of Rhizobium from the nodules
11 To study the cultural, morphological and staining characters of Rhizobium
12 To study the cultural, morphological and staining characters of Azotobacter
13 Enumeration of Azotobacter population in soil by serial dilution pours plate method
14 Isolation of Azospirillum from cereal roots 
15. Enumeration of Cyanobacteria in soil 
16 Study of association between Azolla and Anabaena azollae 
17 Determinations of Actinomycetes population in soil by serial dilution pour plate method
18 Viable counts of fungi in soil by serial dilution pour plate method
19 Isolation of Trichoderma spp. and its testing 
20 Isolation of Rhizobium from Root Nodules and Strain Identification
21 Isolation, purification and culturing of Azotobacter chroococcum 
22 Enumeration of Rhizobia in soil by most probable number (MPN) method
23 Enumeration of Azospirillum by most probable number (MPN) method
24. Estimation of phosphate solubilizing capacity of microorganisms
25 Isolation of plant growth promoting rhizobacteria (PGPR) 
26 Characterization of plant growth promoting rhizobacteria 
27 Isolation, identification, enumeration of spores and inoculums production of AM fungi
28 Glossary